Pretransplant Detection of Human Minor Histocompatibility Antigen-Specific Naive and Memory Interleukin-2-Secreting T Cells Within Class I Major Histocompatibility Complex (MHC)-Restricted CD8 + and Class I1 MHC-Restricted CD4+ T-cel l Subsets
نویسنده
چکیده
Recent studies have shown that host-reactive interleukin2 (IL-2)-secreting donor T lymphocytes (TI) are critically involved in the development of acute graft-versus-host disease (GVHD) after allogeneic HLA-identical sibling bone marrow transplantation (BMT). To further characterize the responding TI, we determined the frequency of pretransplant IL-2-secreting TI-precursors (TI-p) between eight HLA-A, -B, -C, -DR, and -DQ-identical sibling donor-host pairs in both the graft-versus-host (GVH) and the host-versus-graft (HVG) direction. High frequencies of pretransplant host-reactive donor TI-p (1 /I 8,000 to 1 /49,000) were detectable in five patients with grade II acute GVHD. Donor-reactive host TI-p (1/3,700 to 1/31,000) were observed in previously in vivo primed (n = 5) and unprimed (n = 1) patients. In two pairs tested after previous in vivo priming, pretransplant donor-reactive host TI-p were
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